mops minimal media Search Results


minimal mops medium  (Teknova)
95
Teknova minimal mops medium
Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a <t>MOPS</t> <t>medium</t> containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.
Minimal Mops Medium, supplied by Teknova, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mops+minimal+media/MOPS+Minimal+Media+Kit/bio_rxiv__64898__2026__04__01__713023-145-13-16
Average 95 stars, based on 1 article reviews
minimal mops medium - by Bioz Stars, 2026-09
95/100 stars
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isotope supplemented minimal mops media  (Cambridge Isotope Laboratories)
90
Cambridge Isotope Laboratories isotope supplemented minimal mops media
Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a <t>MOPS</t> <t>medium</t> containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.
Isotope Supplemented Minimal Mops Media, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mops+minimal+media/isotope+supplemented+minimal+mops+media/pmc03462262-266-15-20
Average 90 stars, based on 1 article reviews
isotope supplemented minimal mops media - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
mops minimal media  (Cambridge Isotope Laboratories)
90
Cambridge Isotope Laboratories mops minimal media
Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a <t>MOPS</t> <t>medium</t> containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.
Mops Minimal Media, supplied by Cambridge Isotope Laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mops+minimal+media/mops+minimal+media/pmc02854419-483-8-19
Average 90 stars, based on 1 article reviews
mops minimal media - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

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Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a MOPS medium containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.

Journal: bioRxiv

Article Title: Expanding the scope of redox-balance growth coupling techniques with a carbon cofeeding strategy

doi: 10.64898/2026.04.01.713023

Figure Lengend Snippet: Schematic depicting the genetic modifications to enable the overaccumulation of NADPH (Δ pgi , Δ edd , Δ qor , Δ sthA ) and the deletion of aceA to prohibit growth on acetate as a carbon source. Pathways enabling growth on a mixture of acetate and glucose are shown below and highlighted (red: acetaldehyde (strain APEQS_PduP), yellow: 3-HB (strain APEQS_3-HB), blue: mevalonate (strain APEQS_MEV_sa)). b, Simplified metabolic stoichiometries showing only bioavailable carbon and relevant reducing equivalents and assuming all carbon flows to acetyl-CoA (our pathways’ precursor molecule). Simplified stoichiometry of E. coli fermentative metabolism and APEQS metabolism when grown on glucose, showing redox-balanced fermentative or rescue pathways below. Ethanol fermentation requires less acetyl-CoA (green) than is produced from one glucose when redox balanced, reflecting its suitability as a fermentation pathway. Partially reducing pathways consume more acetyl-CoA (red) than is made available per unit glucose when redox balanced, indicating their inability to resolve redox balance in APEQS without acetate co-feeding. c, Unsuccessful growth coupling of strain APEQS_PduP in the absence of acetate (n=3). d, Unsuccessful growth coupling of strain APEQS_3-HB in the absence of acetate (n=3). e, Unsuccessful growth coupling of strain APEQS_MEV_sa in the absence of acetate (n=3). f, Successful growth coupling of strain APEQS_PduP when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). g, Successful growth coupling of strain APEQS_3-HB when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). h, Successful growth coupling of strain APEQS_MEV_sa when the strain is grown with additional 100 mM sodium acetate to satisfy stoichiometric constraints (n=3). All experiments were conducted in a MOPS medium containing 2% glucose with or without 100 mM acetate. Various concentrations of IPTG were added to modulate the induction of the three partially reducing pathways (high [IPTG]: blue (0.5 mM for p15A-based A5c backbone; 0.05 mM for ColE1-based pQE backbone), medium [IPTG]: purple (0.05 mM for p15A-based A5c backbone; 0.005 mM for ColE1-based pQE backbone), no IPTG: red). An empty vector control was included to demonstrate growth without leaky expression (orange). All growth experiments were repeated a minimum of 3 times and showed identical results.

Article Snippet: For growth coupling experiments and strain evolution, bacterial strains were grown in a minimal MOPS medium (Teknova M2106) containing 2% glucose (w/v) supplemented with the appropriate antibiotic based on the plasmid(s) present in the strain.

Techniques: Produced, Plasmid Preparation, Control, Expressing